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Thermo Fisher

Dionex UltiMate 3000

Thermo Fisher Dionex UltiMate family
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Dionex UltiMate 3000 — Inventory photo
Fig. 01Dionex UltiMate 3000Inventory photo[1]

Vacuum

RS Pump[1]

Optics

Column Compartment[1]

How it works

The UltiMate 3000 pump family includes UHPLC, HPLC, and biocompatible pumps, and the pump line includes binary, quaternary, dual-gradient, nano, micro, and semi-preparative variants.[2]

Applications

The UltiMate 3000 pump family is used for nano LC, standard LC, UHPLC, and semi-preparative chromatography.[2]

What do the numbers mean?

Vacuum & pumping6

RS Pump[1]
Accurate?
System components listed
Autosampler, RS Pump, Column Compartment, Tray[1]
Accurate?
Pump family
UHPLC, HPLC, and biocompatible pumps[2]
Accurate?
Pump flow range
20 nL/min to 10 mL/min[2]
Accurate?
Maximum pressure
Up to 1250 bar[2]
Accurate?
RSLCnano maximum pressure
800 bar[3]
Accurate?

Optics & imaging1

Column Compartment[1]
Accurate?

Performance1

RSLCnano sample injection
High-precision sample injections down to 10 nL[3]
Accurate?

Configuration & options6

Autosampler[1]
Accurate?
Tray[1]
Accurate?
Manufacturer
Thermo Scientific / Dionex[1]
Accurate?
Model
UltiMate 3000[1]
Accurate?
RSLCnano flow range
20 nL/min up to 50 μL/min[3]
Accurate?
RSLCnano gradient delay volume
25 nL[3]
Accurate?

Vintage & configurations

Documented models & variants

DesignationGenerationVintageChangesSource
ISO-3100SD———documents.thermofisher.com[4]
ISO-3100BM———documents.thermofisher.com[4]
HPG-3200SD———documents.thermofisher.com[4]
HPG-3200RS———documents.thermofisher.com[4]
HPG-3200BX———documents.thermofisher.com[4]
HPG-3400SD———documents.thermofisher.com[4]
HPG-3400RS———documents.thermofisher.com[4]
LPG-3400SD(N)———documents.thermofisher.com[4]
LPG-3400RS———documents.thermofisher.com[4]
LPG-3400XRS———documents.thermofisher.com[4]
DGP-3600SD(N)———documents.thermofisher.com[4]
DGP-3600RS———documents.thermofisher.com[4]
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What does it need to run?

Site utility requirements, footprint, and infrastructure needed to install and operate this tool. Sourced from public records.

  • ConfigurationRS Pump[1]
  • System components listedAutosampler, RS Pump, Column Compartment, Tray[1]
  • Pump familyUHPLC, HPLC, and biocompatible pumps[2]
  • Pump flow range20 nL/min to 10 mL/min[2]
  • Maximum pressureUp to 1250 bar[2]
  • RSLCnano maximum pressure800 bar[3]

Where are the manuals?

Generated from public-source data on file. Enter your email to access — nothing is published; details are routed privately.

Not publicly documented

Field notes

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Frequently asked questions

What chromatography modes does the UltiMate 3000 pump family cover?

The UltiMate 3000 pump family covers UHPLC, HPLC, and biocompatible pumping, and the family includes nano, standard, and semi-preparative flow ranges.[2]

What kinds of advanced chromatography workflows are supported by the dual-gradient pumps?

The dual-gradient pumps support automated on-line SPE-LC, inverse gradient operation for charged aerosol detection, tandem and parallel analyses, and automated application switching.[2]

Not publicly documented

The following facts about the Dionex UltiMate 3000 are absent from this record as of this revision. First-hand knowledge or a citation closes a gap; every submission is reviewed before publication.

  • No publicly documented production dates or lifecycle milestones (introduction, end of production, EOL) for the Dionex UltiMate 3000 are on record.

    Answerable by: OEM historical records or a trade-press announcement

  • The control-system platform and OS era of the Dionex UltiMate 3000 are not on record.

    Answerable by: an engineer who operated it or OEM installation records

  • No publicly documented failure modes or field errata for the Dionex UltiMate 3000 are on record.

    Answerable by: a field service engineer, process engineer, or maintenance technician

  • The process node or technology generation of the Dionex UltiMate 3000 is not on record.

    Answerable by: an OEM datasheet or a fab qualification report

  • No publicly documented compatible parts, consumables, or accessories for the Dionex UltiMate 3000 are on record.

    Answerable by: an OEM parts catalog or a service engineer

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Sources & citations

Sources (4)Every fact above is drawn from these public sources
  1. [1]Inventory photo
  2. [2]documents.thermofisher.com — documents.thermofisher.comdocuments.thermofisher.com
  3. [3]spectralabsci.comspectralabsci.com
  4. [4]documents.thermofisher.com — documents.thermofisher.comdocuments.thermofisher.com
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Last updated Oct 5, 2026.

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