Leica
SP5
Provisional entry — research in progress
This page was generated automatically from cited public sources and hasn't completed the full research pass yet. Every specification shown carries its source; more detail is added as research completes.
The Leica TCS SP5 is a spectral confocal and multiphoton microscope system from Leica Microsystems. The Leica TCS SP5 is based on the SP spectral detection module, which allows selection of any spectral emission band in the visible range. The Leica TCS SP5 supports up to five confocal channels simultaneously.[1][2]
Plate 012-1 Intro to the Leica SP 5
Paul Siri WilsonWatch on YouTubePlate 022-1 Intro to the Leica SP 5
bizmonkey007Watch on YouTubePlate 032-5 Your First Use of the Leica SP 5.mp4 (w/ Commentary)
bizmonkey007Watch on YouTubePlate 04Part 1: Leica SP5 Training for SP8 Users
NUBiological Imaging FacilityWatch on YouTubePlate 05Leica DMI 6000 CS Inverted Microscope Confocal SP5 DMI6000 B Predecessor to DMi8 [BOSTONIND] - 6981
WWW.BOSTONIND.COM | Laboratory & Scientific Lab Equipment | Boston Industries, Inc.Watch on YouTube
What it is
The Leica SP5 is a laser point-scan confocal microscope used for imaging and inspection work in a microscopy core environment. The Leica SP5 is a point-scan confocal microscope with laser lines at 405, 458, 476, 488, 496, 514, 543, 594, and 633nm, and it supports regular and high-speed resonance scanning modes.[4]
The Leica TCS SP5 is a spectral confocal and multiphoton microscope system. The system is a laser scanning microscope platform intended for confocal imaging and multiphoton imaging.[1]
How it works
The Leica TCS SP5 uses spectral detection with selectable emission bands. The SP detection module allows selection of any spectral emission band in the visible range, five spectral bands can be selected simultaneously, and two of the detectors can operate for fluorescence lifetime imaging microscopy.[1]
Where it fits in the process flow
General reference — not yet source-verifiedThe Leica SP5 sits at the imaging and metrology stage of a laboratory workflow after a sample has been prepared and mounted. The instrument is used to observe fluorescent or otherwise optically active specimens before image analysis or downstream interpretation.
Applications
The Leica SP5 is used for confocal fluorescence imaging in biological research. The instrument is also used for multiphoton imaging, spectral imaging, and fluorescence lifetime imaging microscopy.[1][4]
- inverted confocal microscopy
What do the numbers mean?
Optics & imaging3
- FLIM operation
- Two detectors may operate as a sensor for fluorescence lifetime imaging microscopy (SP-FLIM combination)[1]
Performance1
- Tandem Scanner
- Conventional and resonant scanner in one system; switch by computer controlled super precision mechanical actuator[2]
Control & software1
Configuration & options10
- Spectral band selection
- Any spectral emission band in the visible range; five spectral bands can be selected simultaneously[1]
- Conventional scanner features
- True confocal point-scanning; extra large field of view (21 mm); 64 Megapixel images (8k x 8k); beam park data acquisition; spot-bleaching FRAP; zoom and pan function; slow scan mode[2]
- Resonant scanner features
- True confocal point-scanning; large field of view (15 mm); zoom and pan function; 16,000 lines per second; 250 frames per second; 25 frames per second 512 x 512; up to five confocal channels simultaneously[2]
Where are the manuals?
Generated from public-source data on file. Enter your email to access — nothing is published; details are routed privately.
Not publicly documented
Field notes
No research found yet — worked with this tool? Share what you know.
Frequently asked questions
What scanning modes does the Leica SP5 support?
The Leica SP5 supports regular and high-speed resonance scanning modes.[4]
Not publicly documented
The following facts about the SP5 are absent from this record as of this revision. First-hand knowledge or a citation closes a gap; every submission is reviewed before publication.
No publicly documented production dates or lifecycle milestones (introduction, end of production, EOL) for the SP5 are on record.
Answerable by: OEM historical records or a trade-press announcement
No publicly documented variants, configuration options, or revision breakpoints of the SP5 are on record.
Answerable by: an OEM product catalog or an engineer who ordered or specified the tool
The control-system platform and OS era of the SP5 are not on record.
Answerable by: an engineer who operated it or OEM installation records
No publicly documented failure modes or field errata for the SP5 are on record.
Answerable by: a field service engineer, process engineer, or maintenance technician
The process node or technology generation of the SP5 is not on record.
Answerable by: an OEM datasheet or a fab qualification report
No research found yet — worked with this tool? Share what you know.
Sources & citations
Sources (5)Every fact above is drawn from these public sources
- [1]doi.org — doi.orgdoi.org
- [2]biology.uiowa.edu — biology.uiowa.edubiology.uiowa.edu
- [3]microscopy.duke.edu — microscopy.duke.edumicroscopy.duke.edu
- [4]bumc.bu.edu — bumc.bu.edubumc.bu.edu
- [5]research.uky.eduresearch.uky.edu
Last updated Oct 4, 2026.
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